MONITORING BIOACCUMULATION OF A PROBIOTICS SACCHAROMYCES CEREVISIAE IN ARTEMIA NAUPLII

FOR TILAPIA FINGERLING

Ahmed M. Abdel-Wahab

Fish Health and Management Department, Central Laboratory For Aquaculture Research, Agricultural Research Center, Egypt.

Received 9/ 8/ 2014

Accepted 6/ 9/ 2014

Abstract

Artemia nauplii have been used successfully as biological carriers for transferring essential nutrients to predators. In the present study artemia
was used as bioencapsulate for Saccharomyces cerevisiae and evaluate the immunological effect of this form in tilapia fingerling. Fish was divided into eight groups in three replicates; fed with 0‰, 5‰, 10‰ and 15 ‰ yeast with and without artemia coating. PCV indicate higher significantly in groups T5, T6, T7 and T8 (27.52, 27.62, 27.72 and 27.92) than T1, T2, T3 and T4 (25.60, 25.90, 26.93 and 27.10). Significant increase in the leukocyte count (103/µl) in T6 and T8 (34.66 and 34.40) than T3, T4, T5, T7 and the negative control T1 and T2 (32.12, 32.94, 32.80, 32.60, 30.22 and 30.50). NBT and Lysozyme was significantly higher in T6 and T8. Survival percentages among experimented fish groups challenged with virulent A. hydrophila were 17, 20, 50, 63.4, 66.7, 86.7, 76.7 and 86.7. Humoral immune response was measured by hemagglutination inhibition test and slide agglutination assays two times 15 days interval. The data was illustrated high immune response in T6 and T8.

It could be concluded that coated S. cerevisiae with artemia is more effective than uncoated one that overcomes the problems of its low digestibility of yeast cell walls and its protein content. The best level of S. cerevisiae was fish fed artemia enriched with 10‰ yeast

Key words: Artemia, bioencapsulate, Saccharomyces cerevisiae, immunological effect