SOME BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES IN PROCAMBARUS CLARKII EXPOSED TO ACUTE TOXICITY OF NEEM EXTRACT, NEEMIX

SOME BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES IN PROCAMBARUS CLARKII EXPOSED TO ACUTE TOXICITY OF NEEM EXTRACT, NEEMIX.

Samah M. Abdel-Kader

Plant Protection Research Institute, Agric. Res. Center, Dokki, Giza,
Egypt.

Received 3/ 6/ 2011 Accepted 5/ 7/ 2011

Abstract

The main objective of the present study was to evaluate the acute toxicity of Neemix and biochemical parameters (haemolymph glucose, total protein and triglyceride). The 24h LC₅₀ of Neemix to the adults crayfish, Procambarus clarkii were evaluated. The LC₅₀ values were 431 and 493 ppm for adults male and female, respectively. Adults P.clarkii were subjected to acute lethal concentration (431ppm) of Neemix for 96 h. The results showed a gradual increase in haemolymph glucose within 72h. The total protein levels decreased at the first 48h then followed by a sudden increase after 72 h. While, the triglyceride value decreased only after 24h. The levels of the haemolymph glucose, total protein and triglyceride have reached more or less the control values by the end of experiment. The increase of the basal (regenerative) cells, the extensive mucification of the inner lining of the gland and the hypertrophy of almost all absorptive and secretory cells were the most observed pathological changes.