ULTRA-STRUCTURAL STUDIES ON CUCULLANUS BARBI (BAYLIS, 1923) – ANEMATODE PARASITE OF MORMYRUS KANNUME WITH EMPHASIS ON SEASONAL VARIATION AND BIOREMEDIATION FOR HEAVY METAL IN THE MUSCLES

ULTRA-STRUCTURAL STUDIES ON CUCULLANUS BARBI (BAYLIS, 1923) – ANEMATODE PARASITE OF MORMYRUS KANNUME WITH EMPHASIS ON SEASONAL VARIATION AND BIOREMEDIATION FOR HEAVY METAL IN THE MUSCLES

Gehan I. Abd El-Bar Shagar¹; Salwa Z.A. Arafa²

and Maaly A. Mohamed¹

¹Fish Health and Diseases Depat., Central Laboratory for Aquaculture Research, Agriculture Research Center, Ministry of Agriculture, Egypt.

²Department of Animal – Faculty of Science – Zagazig University.

Abstract

A total of 202 Momyrus kannume specimens were randomly collected monthly from River Nile, Demietta branch at Meet Ghamer region, Dakahleia Governorate, Egypt during the period from April 2012 to March 2013. All collected fish were subjected to clinical, postmortem, and parasitological examinations. Also, based on light of scanning diction microscopically observation of Cuculanus barbi (Nematoda; Cuncullanidae) are described from the intestine of kanumme, Mormyrus kannume, Nile-inhibiting fish species.The prevalence of Cuculanus barbi infestation in Mormyrus kannume was 2.9%. Seasonal prevalence was highest in autumn (6.5%) and the lowest prevalence was in winter (2.3%). Cucullanus barbi is mainly characterized by awide pseudobuccal capsule, the absence of ventral sucker, long sclerotized spicules which measure from 449.1- 441.5µm and Triangular elevated anal opening. Scanning electron microscopy revealed several importances, as previously unreported morphological differences in the number of pre and post-cloacal papillae of the male worm.This result revealed that the occurrence and characteristic features of Cucullanus barbi had indirect relation with evaluated levels of some heavy metals as Fe, Cu, Zn, Mn, Pb, Cd in muscles.