BIO-PHYSIOLOGICAL RESPONSE OF BIOFILTER ALGAL CANDIDATE ULVA SP. TO DIFFERENT NITROGEN
FORMS AND FLUXES
Yasser T.A. Moustafa1; G. Bougaran2;
M. Callier3 and J.P. Blancheton3, 4
1 Central Laboratory for Aquaculture Research, Abbassa, Abou-Hammd, Sharkia, Egypt, ([email protected]), Mobile: 0020 1140 888918.
2 IFREMER, Station de Nantes, Rue de l’Ile d Yeu –B.P.21105, 44311 Nantes Cedex 3, France.
3 IFREMER, Station de Palavas, Chemin de Maguelone, 34250 Palavas Les Flots, France.
4 UMR ECOSYM, USTL, Place Eugène Bataillon, Montpellier, France.
|Received 8/ 10/ 2013||
Accepted 24/ 11/ 2013
The present study was carried out to study the long term effect of different nitrogen forms and concentrations (14.3, 28.6 and 57.1 µM N-NH4 and 214, 2360, and 3700 µM N-NO3) on the photosynthesis and relative growth rate (RGR) of Ulva sp. for five weeks. Nutrient-enriched seawater was supplied at an exchange rate of between 12.6 and 14.4 volumes per day. RGR was determined weekly. Photosynthetic oxygen evolution response was measured using 20 – 25 mg fresh weight seaweed incubated in a DW3 measuring chamber for 56 minutes under different irradiances with light and dark periods alternating every seven minutes. Photosynthetic light-response curves were drawn using 15 photosynthetic oxygen evolution readings normalized to dry weight. Experimental data were fitted with a Haldane model to calculate photosynthetic rate (Pmax), saturation irradiance (Is) and compensation irradiance (Ic). At the experiment termination, the RGR following nitrate addition were higher, but not significantly, than those with the ammonium supplied. The highest RGR was determined at the medium nitrate concentration. The photosynthetic activity of Ulva sp. showed a positive relationship with nitrogen concentrations from both nitrogen forms. The highest significant Pmax was found at the highest nitrate concentration. The lowest ammonium concentration corresponded to the lowest significant Pmax value. No significant differences were found for Is and Ic irrespective of treatment although, in general, ammonium treatments yielded higher Is values than nitrate treatments. The discrepancy between the growth rate results and photosynthetic oxygen evolution is discussed in light of the reproduction activity and temperature effect.
Keywords: Ulva sp., photosynthetic oxygen evolution, relative growth rate, nitrogen forms, nutrients uptake.