CONTRIBUTION ON PSEUDOMONONAS ANGUILLISEPTICA CAUSING PSEUDOMONONAS SEPTICEMIA AMONG CULTURED EEL (ANGUILLA ANGUILLA)
Mohamed .E. Abou El Atta and Ahmed El- Refaee
Fish Health and Management Department, Central Laboratory For Aquaculture Research, Agricultural Research Center, Egypt
Received 4/ 7/ 2012 Accepted 30/ 8/ 2012
Abstract
Pseudomonas anguilliseptica was isolated from naturally infected eel (Anguilla anguilla), showing septicemic picture, clinically the infected eel, suffered from loss of appetite and loss of equilibrium, dullness, sluggish movement, petechial and diffused hemorrhage distributed in different parts of the body and mouth region so may be called the red mouth disease of eel, also, diffused hemorrhage distributed on the lateral and ventral parts of the body and base of the fins, congestion and reddening of the vent and protrusion of genital opening. Internally the infected eel, showed generalized septicemia or severe hyperemic appearance, congestion of the gills, enlarged liver and in some cases showed petechial hemorrhage on its surface, the gall bladder was distended with bile. Congestion of the kidney and spleen, and accumulation of blood tinged exudates in the abdominal cavity. A total 127 isolates from different organs of infected eel (Anguilla anguilla) and according to the morphological, biochemical characters and API 20, Ps anguilliseptica was identified. The higher percentage was from fins, gills, liver, kidney and spleen and with lower percentage from eye and blood. In experimental injection of eel with isolated Ps anguilliseptica in a dose 0.5 ml (5×105 Cfu /ml) showed I/P route was more virulence than I/M rout. Ps anguilliseptica was sensitive to Ciprofloxacin, Oxytetracyclin and Nalidixic acid and resistant to Amilkacin, Kanamycin and Colistin.
