EFFECT OF
CLOVE OIL AND QUANALDINE AS AN ANESTHETIC AGENTS ON SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS IN SILVER CARP (HYPOPHTHALMICHTHYS MOLITRIX) DURING SPAWNING SEASON
Walaa T. El-Ekiaby1 and Adel Fathy El-Saeed2
1Fish Health and Management Department. 2Fish Physiology and hatching Department, Central Laboratory for Aquaculture Research, Agricultural Research Center, Egypt.
| Received 15/ 7/ 2018 |
Accepted 12/ 8/ 2018 |
Abstract
In this study, the anesthetics of interest were clove oil and quanaldine to comparing their anesthetic efficacy and select the most suitable anesthetic for the species silver carp (Hypophthalmichthys molitrix) broodstock. Thirty broodstock silver carp; fifteen female with an average weight of 2.500-2.700 kg and mean total length of 60-65 cm and fifteen male with an average weight of 2.300-2.400 kg and mean total length of 56-58 cm were divided into five groups. Different concentrations of clove oil G1,G2,G3 (5, 7.5 and 10 ml/100l) respectively, quanaldine G4 (2.5 ml/100 l) and control group G5 were used to determine the anesthesia time, recovery time and their impact on reproductive performance during controlled spawning. The effect of two anesthetics on haematological indices and biochemical parameters were studied. Also, the histopathological changes that occurred in some internal organs of different groups were recorded. Results of the current study indicated that using of clove oil at a rate (5 ml / 100 l) was the optimal concentration causing general anesthesia at (6.0 min) and an average recovery time (3min, 3sec) with successful spawning rate (100%) and latency period (9h). No significant differences between values of haematological and biochemical parameters of clove oil and control one, while quanaldine resulted significant increase in glucose and cortisol levels as compared with the control group. The histopathological changes that occurred in some internal organs of different groups were studied.
