EFFECT OF MALACHITE GREEN FUNGICIDE
ON PHYSIOLOGICAL AND BIOCHEMICAL PROFILES IN NILE TILAPIA (OREOCHROMIS NILOTICUS)
Adel M. E. Shalaby*1; Ahmed A. AbdEl-Rahman 1
Maaly A. Mohamed2 and Amany A. Ghareb1
1 Fish Physiology Department
2 Ecological Department, Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt.
* Corresponding author: Adel. M. Shalaby Tel: 01148342902
E- Mail address: [email protected]
|Received 22/ 10/ 2013||
Accepted 5/ 12/ 2013
The 24, 48, 72 and 96-h LC50 for Nile tilapia “Oreochromis niloticus” exposed to malachite green (MG) were determined and found to be 1.38, 0.90, 0.80 and 0.76 ppm respectively.
Nile tilapia “O. niloticus” (40-50 g/fish) was assigned to three group with three replicates each. The first group was kept as control group. The second and third ones were exposed to sublethal concentration (1/8 and ¼ 96-hr LC50) of malachite green.
The haematological and biochemical changes induced in Nile tilapia (O. niloticus) exposed to these sublethal concentration of malachite green (0.095 and 0.19 mg/l respectively) were carried out under labotratory condition for 30 days.
All treatments produced significant fall of erythrocyte count (RBCs), haemoglobin content (Hb) and packed cell volume (PCV). However, blood indices (MCV and MCH) were increased significantly in fish exposed to herbicide. While, the MCHC was decreased significantly in fish exposed to high dose of malachite green.
The plasma glucose content was significant increased mean while, the plasma total protein was significant reduced in fish exposed to all treatments of malachite green. Also, the total lipids was decreased significantly in the exposed fish with malachite green .The activity of aspartate aminotransferes (AST) and alanine amino transferase (ALT) were also significantly increased in fish exposed to subleathal dose of the tested fungicide.
Key words: Nile tilapia, malachite green, haematology, Biochemistry changes.