EFFECT OF QUINALDINE SULFATE, CLOVE OIL AND TRICAINE METHANESULFONATE ANESTHESIA ON PHYSIOLOGICAL, BIOCHEMICAL PARAMETERS AND MORTALITY OF NILE TILAPIA (Oreochromis niloticus)
Elsayed A.M. Shokr and Fathy, A.
Fish Physiology Department, Central Laboratory for Aquaculture Research, Abbassa, Abo-Hammad, Sharkia, Egypt.
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Received 2/ 10/ 2018 |
Accepted 4/ 11/ 2018 |
Abstract
The present study was carried out to evaluate and compare between the Quinaldine Sulfate, Clove Oil and tricaine methanesulfonate (MS-222) anesthetics and their effect on Nile tilapia (Oreochromis niloticus). After anesthesia, plasma glucose, cortisol, cholesterol (CHOL) and triglyceride (TGL), AST, ALT, total protein, uric acid and creatinine were measured and they are stress responses in the Nile tilapia (Oreochromis niloticus) and modulation of these responses by quinaldine sulfate, clove oil and MS-222 anesthesia. There were dose-related increases in plasma levels of glucose, cortisol, cholesterol (CHOL) and triglyceride (TGL), AST, ALT, total protein, uric acid and creatinine due to stress responses in the Nile tilapia (Oreochromis niloticus) after 24 and 48 hrs. with continuous exposure to quinaldine sulfate, clove oil and tricaine methanesulfonate. Results showed that there was a significant difference between values of glucose, cortisol, cholesterol (CHOL) and triglyceride (TGL), AST, ALT, total protein, uric acid and creatinine in each treatment in compare to control group. Clove oil, quinaldine sulfate and MS-222 make changes in serum glucose, cortisol, cholesterol (CHOL) and triglyceride (TGL), AST, ALT, total protein, uric acid and creatinine levels in Nile tilapia (Oreochromis niloticus). and there is significantly higher circulating in the glucose, cortisol, cholesterol (CHOL) and triglyceride (TGL), AST, ALT, total protein, uric acid and creatinine levels after exposure to clove oil, quinaldine sulfate and MS-222 than control group. All fish in the present study were died in the control group but 30 % of fish that exposed to clove oil were died and 55% of fish exposed to quinaldine were died but 70 % of fish exposed to MS-222 were died. On the other hand, the recovery times in the groups that treated with clove oil 7-9 min., quinaldine 6-8 min. and MS-222 anesthesia is 5-7 min. respectively. The results showed that Physiological and biochemical parameters of Nile tilapia (Oreochromis niloticus) are affected by different concentrations of quinaldine sulfate, clove oil and MS-222 anesthesia. Histological examination of the fish following anaesthesia revealed sporadic ectasia in gill lamellae 24 h after anaesthesia in of fish. No histopathological changes were demonstrated in liver. Results of the examinations suggest that the use of quinaldine sulfate, clove oil and MS-222 does not cause irreversible damage in Nile tilapia. The clove oil is better than quinaldine sulfate and MS-222 anesthesia to Nile tilapia (Oreochromis niloticus).
Key words: quinaldine sulfate, clove oil, MS-222, biochemical profile, histology.
