EFFECTIVITY OF Myxobolus koi SPORES PROTEIN AS IMMUNOSTIMULANT TO PREVENT THE MYXOBOLUSIS IN CARP (Cyprinus carpio
L.)
Gehad A. Ismail1 and M.S. Mohamed 2
1Fish Diseases and Management research Department, Animal Health Research Insti-tute, Agriculture Research Center, Egypt.
2Feeding Department- Central Laboratory for Aquatic Research, Agriculture Research Center, Egypt.
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Received 1 /2 /2021 |
Accepted 25 /2 /2021 |
ABSTRACT
The research was aimed to isolate the whole protein from Myxobolus koi spores from common carp (Cyprinus carpio L.) by SDS-PAGE method. In addition to, analyze its effects on fish immune response and survival rate as feed supplementation. In the present study, 120 fish with average body weight (10.44 ± 0.22g) and average total body length (8.8 ±0.13cm) were divided into four groups (I,II,III,IV). Fish were treated with (1μg protein/ gram fish and/ or 80 M. koi spores/fish). The result showed that there were four bands of whole spore protein with molecule weight (MW) 120 kDa, 86 kDa, 72 kDa and 48 kDa. The result showed total leukocyte counts, total protein and globulin was significant (P<0.05) higher in fish of group II, III and IV as compared with control group. Survival rate increased from 23% to 65% in group II (80 spores/M. koi) and III (1μg protein / gram fish+80 spores/ M. koi.), respectively. We can conclude that, since the molecular weight of M. Koi protein spores is > 10kDa, it could be considered as an immunogenic molecule. In addition to, we our study proved that, M. Koi protein spores can used as immunostimulant as increasing leucocyte count and globulin and can decrease common carp (Cyprinus carpio L.) death by Myxobolusis.
Key words:
SDS-PAGE, Myxobolus koi, Cyprinus carpio
L., Survival rate, Total protein, Gloulin, Leukocytes.
