EFFECTS OF Salvia officinalis EXTRACT ON FISH HEALTH AND REPRODUCTIVE PERFORMANCE OF NILE TILAPIA (Oreochromis niloticus)
Doaa A. El-Araby1; Eman M. Zaki2
and Waheed E. Mohammed2
1Fish Health and Management Dept., 2Reproductive Physiology and Hatchery Department. Central Laboratory for Aquaculture Research (CLAR), Abbassa, Sharkia, (ARC), Egypt.
This study was carried out to evaluate the effect of Salvia officinalis extract on reproductive performance and fish health. S. officinalis was collected from harplest and extracted using ethanol 95%, purified and identified using GC-mass techniques. S. officinals extract was tested as antibacterial invitro and added to commercial diet 30% protein in four treatments (0.0, 0.5, 1.0 and 1.5% diet). Total number of 96 apparently healthy Nile tilapia broodstock was collected and selected from Abbassa Fish Farm for reproduction and divided into four groups in three replicates (each 6 females and 2 males). All fish groups were fed diet twice per day for 5 days per week until satiation for 90 days.
At the end of feeding experiment the number and weight of eggs and fries were occurred. Blood and serum were collected for blood and physiological parameters and the fish was challenged with Aeromonas hydrophila.
The results indicated that S. officinals extract had antibacterial effect against A. hydrophila in vitro and the active principal identified as C10 H18 O Eucalyptol. S. officinals extract had good effect on the weight and number of eggs, fries and embryonic development at 0.5 and 1.5%. 0.5 % of S. officinalis extract was the best treatment which improved fish health by increasing WBCs count, Hb value, albumin, globulin and total protein. Also it increased fish resistance against A. hydrophila.
Key words: Salvia officinalis, Nile tilapia, physiological parameters, Aeromonas hydrophila, challenge, reproductive performance