EFFICACY OF WARM WATER, SODIUM HYPOCHLORITE AND TRISODIUM PHOSPHATE ON E.
Coli O157: H7
AND Salmonella typhimurium ARTIFICIALLY INOCULATED

IN Oreochromis niloticus FILLETS

Ayman M. Sharaf Eldin1*; Mohamed A. Hussein2;

Mohamed A. Elshater 3 and Elsaid A. Eldaly2

1Fish Quality Control and Processing Department, Central Laboratory of Aquaculture Research, Abbassa, Egypt.

2Food Control Department, Faculty of Veterinary medicine, Zagazig University. Egypt.

3Food Safety Department, Animal Health Research Institute, Dokki, Giza. Egypt.

*Corresponding author. Email: [email protected]

Abstract

The Nile tilapia (Oreochromis niloticus) (Tilapia nilotica) is a native fish in Egypt and it is widely consumed as it represent a great protein source. The health of Egyptians is badly affected by some foodborne pathogens such as E. coli O157: H7 and Salmonella Typhimurium which are being transmitted by eating the contaminated Oreochromis niloticus
fillets. This experiment was carried out to decontaminate tilapia fillets samples by reference strains of E. coli O157: H7 and Salmonella Typhimurium. This was done by dipping of samples in warm water (45°C) for 5 minutes, trisodium phosphate 8% for 20 minutes and sodium hypochlorite 50 ppm for 20 minutes. The count of examined E. coli O157: H7 was decreased by 98.05%, 94.5% and 70.49% for previous treatments respectively while the count of Salmonella Typhimurium was decreased by 82.62%, 78.12% and 32.39% for previous treatments respectively. Some fish products with inferior quality were distributed in markets so the government must apply strict legislations on producers to ensure safe fish products for people, and must improve hygienic conditions during fishing, transportation, processing and storage of fish products.

Key words: Foodborne pathogens, E.coli, Salmonella, Oreochromis niloticus, warm water, decontaminators.