EVALUATION OF TAMOXIFEN

TO INDUCE MONOSEX IN NILE TILAPIA (OREOCHROMIS NILOTICUS) REARED IN HAPAS SUSPENDED IN CONCRETE PONDS

Ahmed A. Abd El-Rahman1; Ashraf A. Ramadan2

And
Ahmed S. Diab3

1 Reproductive Physiology And Hatchery; 2 Genetics And Breeding Department; 3 Fish Health Department, Central Laboratory For Aquaculture Research, Agricultural Research Center. Egypt.

Received 2/ 1/ 2012 Accepted 20/ 1/ 2012

Abstract

The present study aimed to replace 17α- methyltestosterone hormone by tamoxifen to produce all male Nile tilapia and investigate final weight, male % and survival rate of Oreochromis niloticus fry treated with 60 mg / kg diet of 17α- methyltestosterone (MT), tamoxifen (100 & 200 mg / kg diet) and control fish. Fish fry reared in hapas suspended in concrete ponds.

The results indicated that there were highly significant differences in final weight, male percentage (%) and survival rate of fry at all treated groups when compared to the control group. The finial weight and male percentage were high significantly to (7.03 ± 0.83, 5.07± o.15, 4.77±0.23 (g) and (92.67±0.58, 81, 67 ±0.49 & 85.33± 0.91 (%) in fish treated with 17α-methyltestosterone, 100 & 200 mg of tamoxifen (antiestrogen) / kg of diet ) respectively than in the control group (4.07+ 0.25 (g) and 60.33±0.61%). While, increased significant in survival rate for control (92.00±0.46%) than fry treated with 17α- methyltestosterone, tamoxifen (antiestrogen) 100 & 200 mg / kg diet were 88.97±0.45, 84.13±0.50 and 77.93±0.60 % respectively, after 105 days. That is to say, using of tamoxifen
200 mg/kg diet for male sex reversal of Oreochromis niloticus fry better than hormone of 17α-methyltestosterone because it was best and safety for human.

Key words:
Oreochromis niloticus, Monosex, Tamoxifen, 17 α-methyltestosterone.