Skip to the content
EVALUATION OF THE POTINTIAL EFFECT OF CLOVE (EUGENIA AROMATIC) OIL IN COMBATING THE AEROMONAS SOBRIA INFECTION IN AFRICAN CATFISH (CLARIAS GARIEPINUS L.)
1Alshimaa A. Khalil; 1Abd elhakim, Y. and 2
Haytham A. Ali
1Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University.
2Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University.
|Received 5/ 1/ 2016
Accepted 26/ 1/ 2016
The present study was conducted to evaluate the pathogenicity of Aeromonas sobria and its ability of producing infections and mortality in African catfish Clarias gariepinus L (Experiment I) and to estimate the potential effect of clove oil as a prophylactic measure against A. sobria infection (Experiment II). One hundred and eighty apparently healthy African catfish (C. gariepinus) weighting 30±5g, collected from Abbassa fish farm, Alsharkia governorate, Egypt. Experimental Clarias gariepinus exhibited aggressive clinical signs and gross lesions began after 12 hrs. post inoculation (PI) of A. sobria that ensure the bacterial capacity to induce the disease. Results suggested the beneficial use of clove oil against A. sobria infection in C. gariepinus. In which the mortalities of C. gariepinus reached 20 % in 1st group which subjected to clove oil by a dose of (1.3μl/L) as a bath for 2 weeks then experimentally infected Intraperitoneally by a dose of 0.2 ml (2.5×108 CFU.ml-1 of A. sobria), however the 2nd group showed about 100% mortalities. While the 3rd and 4th groups showed no mortalities. Results of serum biochemical analysis of using of clove oil as a prophylactic treatment (1st group) is relatively improveme the levels of serum IgM and hepatic reduced glutathione levels (GSH), beside decrease the serum levels of ALT, AST, urea, creatinine and serum cortisol levels with reduction in the hepatic MDA when compared with 2nd group.
Keywords: Clove oil, prophylactic measure, serum biochemical assay, Aeromonas sobria, Clarias gariepinus.