IMMUNOLOGICAL AND PATHOLOGICAL STUDIES ON OREOCHROMIS NILOTICUS FED ON CINNAMON AND OXYTETRACYCLINE IN DIET AND CHALLENGED WITH CLOSTRIDIUM PEREFRINGES.

IMMUNOLOGICAL AND PATHOLOGICAL STUDIES ON OREOCHROMIS NILOTICUS FED ON CINNAMON AND OXYTETRACYCLINE IN DIET AND CHALLENGED WITH CLOSTRIDIUM PEREFRINGES.

Walaa El-Houseiny*; Alshimaa A. Khalil*

and Rehab E. Mowafy**

*Dept. of Fish Diseases, Faculty of Veterinary Medicine, Zagazig University

** Dept. of Pathology, Animal Health Research Institute (Zagazig Branch).

Abstract

The present study was aimed to study the effect of adding cinnamon and oxytetracycline (OTC) to fish diet as a control strategy to illustrate their impact on immunity, growth and histopathological changes of Oreochromis niloticus. A total number of 90 of Oreochromis niloticus with an average body weight of 24.5 ±0.4g were used. The fish were randomly divided into three equal triplicate groups (Each replicate contained 10 fish). Fish were primarily divided into two experimental studies. Experiment I (pre challenged experiment) in which fish was fed with 0.5% cinnamon and 0.5% OTC. The fishes were fed for 2 months and then applied the experiment II (post challenged experiment) the groups after that kept for 15 days observation period. The obtained results revealed that the fish fed diet contained o.5 % cinnamon had a higher final body weight, body gain and body gain % as compared to fish fed OTC dietary treatment. Immunological parameters estimated in this work (IgM and lysozyme) were significantly improved due to supplementation of the cinnamon in compared with that of OTC and control groups. Congestion of most internal organs, deposition of melanomacrophages and hemosiderin, leucocytic cells infilterating most of the parenchymatous organs and some degenerative changes were the obivious pathological changes in post infected control group, these lesions were less demonstrated in both treated groups with cinnamon and oxytetracyclin after challenged with bacteria.