STIMULATION SPAWNING OF COMMON CARP, GRASS CARP AND SILVER CARP BY CARP PITUITARY EXTRACT, HUMAN CHORIONIC GONADOTROPHIN, RECEPTAL AND OVAPRIM HORMONES FOR COMMERCIAL PURPOSES

Mohamed E. Farag1; Mohamed M. Zeinhom2

and Ibrahim H. Ibrahim1

1Fish Genetics & Breeding Department, 2Fish Nutrition Department, Central Laboratory for Aquaculture Research, Abbassa, Agriculture Research Center, Egypt.

Corresponding Author: E-Mail Address: [email protected]

Abstract

One of the most important problems in commercial cyprinid aquaculture is difficulty of obtaining good quality gametes. For this purpose, hormonal treatment is used for stimulating of gametes maturation in commercial-cyprinid production. In the first week of April, common carp were spawned. Each tank contained 5 females and 5 males of common carp (Cyprinus carpio). In the second week of April, 4 females and males of silver carp (Hypophthalmichthys molitrix) were spawned and in the third week of April, grass carp (Ctenopharyngodon idella), 4 females and 4 males were spawned. Fish were divided into five trial groups for treatments. First group (G1) was induced by carp pituitary extract (CPE), second group (G2) was injected with mixture of human chorionic gonadotrophin (HCG) and (CPE), third group (G3) was injected with (HCG) alone, forth group (G4) was injected with Ovaprim hormone (OVP) and fifth group (G5) that was injected with (LH-RH) luteinizing hormone-releasing hormone analogues “Receptal” Buserelin Acetate (BA). The results showed that the hours of latency time in common carp were relatively shorter than both of grass and silver carp for all treated groups while, in silver carp the latency time was the longest. The hatching rate percentages of G1 showed the highest ratios; 50, 49 and 48% followed by G5 in common carp, grass carp and silver carp, respectively. The weight of eggs showed significance differences (P<0.05) among the five treatments. The highest masses of eggs were presented in G1, G4 and G5 and the lowest masses of eggs were presented in G3 for common, grass and silver carp, respectively. However, a total cost was higher in G4 and G5 than G2 and G3 while, the lowest cost was found in G1 in common, grass and silver carp. Regarding the economic efficiency, CPE showed higher value followed by receptal for all carp species and common carp showed the highest value for carp pituitary extract.

Keywords: Stimulation spawning, Carp, Reproductive performances, Carp pituitary extract, Human chorionic gonadotrophin, Receptal (buserelin acetate), Ovaprim hormone.