STUDY ON VIBRIOSIS IN MUGIL CAPITO IN EL-DAKAHLIA AND DAMITTA GOVERNORATES, EGYPT

STUDY ON VIBRIOSIS IN MUGIL CAPITO IN EL-DAKAHLIA AND DAMITTA GOVERNORATES, EGYPT

El-Bouhy Z.¹; El-Nobi, G.¹; Abd elhakeem El-Murr¹

and Samar Abd El-Hakim²

¹Department of Fish Diseases and Management, Faculty of Vet. Medicine, Zagazig University, Egypt.

²Animal Health Research Institute, Mansoura, Egypt.

Abstract

Vibriosis is considered the most important threatening disease problem facing aquaculture specially Mugil capito (Liza ramada). This study aimed to conduct bacteriological study on Vibriosis in different fish farms in El-Dakahlia and Damitta governorates. The isolated bacteria on tryptic soya agar (TSA) with 2% sodium chloride gave circular creamy colored colonies. On thiosulfate citrate bile salt sucrose agar (TCBS) gave yellow colonies for Vibrio alginolyticus and gave green colonies for Vibrio parahemolyticus. PCR yielded a single specific and clear amplificated band of expected size 737Bp and 387Bp for V. alginolyticus and V. parahemolyticus respectively. The total prevalence of Vibriosis infection in Mugilcapito in study was 56.11%.The highest infection rate was recorded during summer season with prevalence rate 88.88% followed by spring 63.63%, winter 45.90% and the lowest was during autumn with prevalence rate 37.50%.Total prevalence of Vibrio alginolyticus among naturally infected Mugil capito was 69.76%. While Vibrio para hemolyticus in Mugil capito was 30.24%.