USED OF ZELOITE TO REDUCE THE IRON TOXICITY FOR ENHANCEMENT THE GROWTH PERFORMANCE AND PHYSIOLOGICAL STATUS IN NILE TILAPIA (Orechromius nloticus).
Adel M. Shalaby1; Eman A.A. Abdel-Hamid3;
Mohamed k. Khames2; Adel Fathy1 and Amany A. Gharieb1
1hatchery and fish physiology, 2fish limnology, 3fish nutrition department. Central Laboratory for aquaculture research, Abbass, Sharkia.
Corresponding auther: [email protected].
Received 19 /1 /2021
Accepted 21 /2 /2021
Iron is an essential element involved in many vital physiological functions in fish. Excess of iron concentration caused some toxic effects. The present study aims to evaluate the possible ameliorative effect of Zeolite against iron toxicity in Nile tilapia . Tilapia fry were divided into five groups. The 1st group (lefet as control group without any addition. The 2nd , exposed to 75mg/L of ferrous oxide. While 3rd , 4th and 5 th groups exposed to same concentration of ferrous oxide (Fe2O3) with 2, 4 and 8g of Zeolite/L, respectivelyy .The growth performance of O. niloticus, fry improved with the high concentration of zeolite compared to Fe group alone and control group. The best feed conversion ratio was recorded in the fish fingerlings exposed to 8g of zeolite/L compared with the control After exposure fish to Fe a reduction significantly (P<0.05) in erythrocyte count (RBCs) haemoglobin content (Hb), haematocrit value (Hct). These parameters were improved when zolite was applied with Fe. The values of (RBCs), Hb and Hct were increased significantly to be as in the control fish group. Similarly, there were significant decreased in total protein (TP) in plasma of fish exposed to iron alone and iron with low doses of zeolite. While, these values were improved in fish after addition of 8 g of zeolite/ L. Whereas, plasma glucose concentration, total lipid, cholesterol, uric acid and creatinine were increased significantly in fish exposed to Fe alone and mixture of same dose of Fe with low of zeolite. Similarly, alanine aminotransferase (ALT) and alkaline phosphatase (ALP), were increased significantly in fish exposed to Fe alone.
Addition of zeolite to Fe contaminated medium enhanced these biochemical parameters in fish and the enzyme activities returned to be as control group. Addition of zeolite to Fe contaminated medium considerably reduced Fe absorption and accumulation in muscle and gills.
Nile tilapia, Fe, Zeolite, growth performance, Physiological parameter.