Ahmed M. Abdel-Wahab

Fish Health and management Department. Central Laboratory for Aquaculture Research, Abbassa, Agriculture Research Center, Egypt.

Received 9/ 1/ 2018

Accepted 25/ 2/ 2018


The frequency of viral disease outbreaks in the shrimp aquaculture industry has markedly limited the development of shrimp aquaculture in the world. In the present study, primary tissue culture from Penaeid semesulacatus used for virological studies of diseased shrimp. A total of 70 adult symptomatically diseased shrimp of common Penaeid species were collected from El-Adabia,in Suez governorate. Gills, lymphoid organ, cuticular epithelium shrimp, and subcutecular connective tissue were prepared for isolation and histopathological examination which showed evidences of diseased agent invasive. On the tissue culture it was showed cytopathic effect developed by 48 hrs to 96 hrs post inoculation in 17 samples out of 70 samples as total collection. For more investigation harvested fluid from the tissue culture was negatively stained with phosphotungstic acid and examined by electron microscope that revealed presence of virus-like particles.

It can be concluded that, the wild shrimp isn’t safe, can car rymany diseases and should be examined before used as breeder in the hatcheries.

Key words: Penaeid, shrimp and cell culture.